Sun exposure is the cause of pigmentation disorders and skin aging.  The initial reaction to UVB radiation is mostly dependent on TNF-α overexpression. Caspases 3 can be used to identify apoptosis in the most afflicted basal cell layer. Malonyl dialdehyde (MDA) is one of the non-toxic metabolite chemicals produced by lipid peroxidation brought on by UV radiation. Reactive oxygen species levels raise the synthesis of melanin and quicken the process of pigmentation. The most recent focus on the xanthone found in mangosteen peel (Garcinia mangostana Linn.) is as an antioxidant. The aim of this study is to assess the benefits of ethyl acetate extract from mangosteen peel cream in mitigating skin pigmentation by determining its antioxidant effects on MDA, TNF-α, and caspase 3 levels. At the Wates Veterinary Center Yogyakarta, 25 guinea pigs were split into five control groups for an experimental study, which used a post-test only control group design. The control group, not receiving any therapy. Group induction, subjected to UVB radiation. The group in negative control was subjected to UVB rays and was given cream base. The test group received 12% ethyl acetate extract of mangosteen peel cream in addition to UVB exposure. The positive control group received 5% hydroquinone cream application and UVB exposure. The amount of epidermal melanin by Masson fontana staining, skin brightness by mexameter, and the assessment of MDA serum by TBARS, TNF-α, caspase 3, and tyrosinase enzyme by ELISA. ANOVA and Kruskal Wallis tests in statistical analysis show significance at p < 0.05.

Results: The ethyl acetate extract of mangosteen peel cream decreased the levels of all variables significantly. 

Conclusion: Following UVB exposure, the guinea pigs' back skin were brightened and the amounts of MDA, TNF-, caspase 3, and epidermal melanin were decreased by the mangosteen peel cream's ethyl acetate extract.